Level 2 Semester 2 Fisheries FTL204 Lab pdf-- L2 S2 [FISH]

note : here 2 pdf

 

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                                click here to download link 2

 

 

 

 

 FTL 2 2 lab sheet....

Experiment No. 1

General Suggestions and Safety in a Microbiology Laboratory

1-6-10-7

Laboratory philosophy

Iaboratories are placesfor practical work by professional scientists, technicians and students. All to enter should wear laboratories coats, stout shoes and often safety spectacles, Recreational and refreshment areas should be provided away from the laboratory. Smoking, eating, drinking and application of cosmetics should not be allowed in the laboratory. Food and drink for consumption should never be brought to the laboratory. In short, laboratories are specialized areas that require careful planning, sufficient resources and rigorous supervision.

General Suggestions

(1) Before each laboratory period, read over the exercise to be done and plan your works carefully and know how exercise to be done. Few trials should be done before

commencement of the actual experiment. (2) Each laboratory meeting will begin with a short discussion and instruction period. Do not begin work until you have received your instruction.

(3) Properly record all observations at the time when they are made. Laboratory examination

will cover both information given by your laboratory teacher and that contain in the manual as well as your own observation and education. Drawing should be made where it is necessary.

Safety in the Microbiology Laboratory

(1) Out door cloths and bags should be left outside the laboratory, preferably in a locker,

laboratory coats and aprons should be worn at all time in the microbiology laboratory.

(2) Top of the laboratory desk must be sponge off with germicide solution at both the

beginning and close of each laboratory period.

(3) Eating, drinking or smoking inside the laboratory is strictly prohibited. Cuts and abrasions should be covered with a waterproof dressing. (4) Work in a microbiology laboratory should not be done without supervision. (5) A safety bulb should always be used during transferring liquids, mouth pipetting is

prohibited.

(6) Contaminated pipettes should be placed in to discard jar-containing disinfectant. (7) If contaminated materials are accidentally spilled on to the bench or floor, it should be

disinfected with an appropriate disinfectant.

(8) Any type of accident like cuts, abrasion or breaking of glassware with bacterial culture

should be reported to class teacher or supervisor.

(9) Contaminated materials should be placed on the designed place. (10) Hands should be washed after being completion of work with disinfectant solution in

the laboratory. The work and working placed should be neat and clean.

Important Terminology Used in Microbiology Laboratory

Sterilization

The complete destruction or removal of all living microorganisms or their spores by any

physical, chemical or mechanical means is called sterilization.

(a) Sterilization by Heat

Heat sterilization can be accomplished by several ways: (1) Red heat incineration

Instruments such as inoculating loop, wire etc. are sterilized by holding them in flame of spirit lamp of Bunsen burner until they are red-not. (ii) Flaming

It is a method that commonly used for decontamination of the mouth of bottles, flasks,

tubes glass slides ate bun